Rotavirus detection by dot blot hybridization assay using a non-radioactive synthetic oligodeoxynucleotide probe
نویسندگان
چکیده
منابع مشابه
Development of a recombinant protein-based dot-blot hybridization assay for the detection of antibody to chicken infectious bronchitis virus
Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, inducesstrong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of IBV antibody.Initially, 72 serum samples were tested for the presence of IBV antibody using a c...
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A dot blot hybridization immunoenzymatic assay for the rapid detection of cytomegalovirus DNA in urine samples was developed by using a digoxigenin-labeled probe which was immunoenzymatically visualized by antidigoxigenin Fab fragments labeled with alkaline phosphatase. A total of 516 urine samples from different groups of subjects were analyzed, and the hybridization assay was able to yield re...
متن کاملDot hybridization assay for distinction of rotavirus serotypes.
We have developed a hybridization assay that permits distinction of rotavirus serotypes 1, 2, 3, and 4. The serotype of rotaviruses from stool samples or tissue culture was recognized by hybridization of specific probes to (i) blots of viral double-stranded RNAs electrophoresed in agarose gels (Northern blots) or (ii) heat-denatured double-stranded RNAs directly dotted on nylon membranes. The p...
متن کاملChemiluminescence dot blot hybridization assay for detection of B19 parvovirus DNA in human sera.
A chemiluminescence dot blot hybridization assay was used for the detection of B19 parvovirus DNA in human sera by using digoxigenin-labeled probes. The probes were revealed immunoenzymatically by use of anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. The chemiluminescence signal was obtained by reacting the labeled probe-target complex with an enzyme-triggerable dioxetane ...
متن کاملdevelopment of a recombinant protein-based dot-blot hybridization assay for the detection of antibody to chicken infectious bronchitis virus
nucleocapsid (n) protein of infectious bronchitis virus (ibv), one of the viral structural proteins, inducesstrong antibody response in natural infection. in this study, a simple, recombinant n protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of ibv antibody.initially, 72 serum samples were tested for the presence of ibv antibody using a c...
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ژورنال
عنوان ژورنال: Epidemiology and Infection
سال: 1992
ISSN: 0950-2688,1469-4409
DOI: 10.1017/s0950268800049621